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| Registros recuperados: 91 | |
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| Reveillon, Damien; Sechet, Veronique; Hess, Philipp; Amzil, Zouher. |
| The neurotoxin β-N-methylamino-L-alanine (BMAA) is naturally present in some microalgal species in the marine environment. The accumulation of BMAA has widely been observed in filter-feeding bivalves that are known to consume primary producers constituting the base of complex aquatic food webs. This study was performed to assess the occurrence of BMAA and isomers in mollusks collected from nine representative shellfish production areas located on the three French coasts (Channel, Atlantic and Mediterranean sites). The use of a highly selective and sensitive HILIC-MS/MS method, with D5DAB as internal standard, revealed the systematic detection of BMAA and DAB, in concentrations ranging from 0.20 to 6.7 μg g-1 dry weight of digestive gland tissues of... |
| Tipo: Text |
Palavras-chave: BMAA; Chaetoceros; Thalassiosira; Phaeodactylum; Bivalves; Seafood. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00297/40780/39784.pdf |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tectus niloticus; Gastropods; Gambierdiscus polynesiensis; QPCR assays; CBA-N2a; LC-MS; MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00426/53798/54719.pdf |
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| Tong, Mengmeng; Kulis, David M.; Fux, Elie; Smith, Juliette L.; Hess, Philipp; Zhou, Qixing; Anderson, Donald M.. |
| For many years, the study of toxic Dinophysis species was primarily restricted to field populations until it was recently demonstrated that some of these organisms can be mixotrophically cultured in the laboratory with the ciliate prey, Myrionecta rubra. which had previously been fed with cryptophytes of the genus Teleaulax and Geminigera. Here we investigated the influence of growth phase and light intensity on the production of diarrhetic shellfish poisoning (DSP) toxins and pectenotoxins (PTXs) in cultures of Dinophysis acuminata from the northeastern United States. The cell toxin content of okadaic acid (OA), dinophysistoxin-1 (DTX1), pectenotoxin-2 (PTX2), and the okadaic acid diol ester (OA-D8) varied significantly with growth phase under all light... |
| Tipo: Text |
Palavras-chave: Diarrhetic shellfish poisoning (DSP); Dinophysis acuminata; Dinophysistoxin (DTX); Growth phase; Light; Okadaic acid (OA); Pectenotoxins (PTXs). |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00037/14827/12195.pdf |
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| Salas, Rafael; Tillmann, Urban; John, Uwe; Kilcoyne, Jane; Burson, Amanda; Cantwell, Caoimhe; Hess, Philipp; Jauffrais, Thierry; Silke, Joe. |
| Azaspiracids (AZAs) are a group of lipophilic polyether compounds first detected in Ireland which have been implicated in shellfish poisoning incidents around Europe. These toxins regularly effect shellfish mariculture operations including protracted closures of shellfish harvesting areas for human consumption. The armoured dinoflagellate Azadinium spinosum Elbrachter et Tillmann gen. et sp. nov. (Dinophyceae) has been described as the de novo azaspiracid toxin producer; nonetheless the link between this organism and AZA toxin accumulation in shellfish has not yet been established. In August 2009, shellfish samples of blue mussel (Mytilus edulis) from the Southwest of Ireland were analysed using liquid chromatography-tandem-mass spectrometry (LC-MS/MS) and... |
| Tipo: Text |
Palavras-chave: Azaspiracids; AZP; AZA toxins; Biodeposits; Dinoflagellates; Feeding experiment; Ireland; LC-MS/MS; Mussels; Phylogeny; Taxonomy. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00049/16049/15836.pdf |
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| Roue, Melanie; Darius, Helene Taiana; Ung, Andre; Viallon, Jerome; Sibat, Manoella; Hess, Philipp; Amzil, Zouher; Chinain, Mireille. |
| Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were... |
| Tipo: Text |
Palavras-chave: Giant clams; Ex situ exposure to toxic algae; Ciguatoxins; Gambierdiscus polynesiensis; Anatomical distribution; Toxin elimination; CBA-N2a; LC-MS/MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00440/55123/56579.pdf |
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| Pisapia, Francesco; Holland, William C.; Hardison, D. Ransom; Litaker, R. Wayne; Fraga, Santiago; Nishimura, Tomohiro; Adachi, Masao; Lam Nguyen-ngoc,; Sechet, Veronique; Amzil, Zouher; Herrenknecht, Christine; Hess, Philipp. |
| Species in the epi-benthic dinoflagellate genus Gambierdiscus produce ciguatoxins (CTXs) and maitotoxins (MTXs), which are among the most potent marine toxins known. Consumption of fish contaminated with sufficient quantities of CTXs causes Ciguatera Fish Poisoning (CFP), the largest cause of non-bacterial food poisoning worldwide. Maitotoxins, which can be found in the digestive system of fish, could also contribute to CFP if such tissues are consumed. Recently, an increasing number of Gambierdiscus species have been identified; yet, little is known about the variation in toxicity among Gambierdiscus strains or species. This study is the first assessment of relative CTX- and MTX-toxicity of Gambierdiscus species from areas as widespread as the... |
| Tipo: Text |
Palavras-chave: Ciguatera fish poisoning; Gambierdiscus; Ciguatoxins; Maitotoxins; Neuro-2a assay; Erythrocyte lysis assay. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00374/48552/49411.pdf |
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| Darius, Helene Taiana; Roue, Melanie; Sibat, Manoella; Viallon, Jerome; Gatti, Clemence Mahana Iti; Vandersea, Mark W.; Tester, Patricia A.; Litaker, R. Wayne; Amzil, Zouher; Hess, Philipp; Chinain, Mireille. |
| The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic... |
| Tipo: Text |
Palavras-chave: Ciguatera poisoning; Ciguatoxins; Tripneustes gratilla; Sea urchin; Echinoidea; Gambierdiscus polynesiensis; Windowscreens; Artificial substrates; QPCR assays; CBA-N2a; LC-MS/MS. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00436/54716/56153.pdf |
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| Abadie, Eric; Muguet, Alexia; Berteaux, Tom; Chomerat, Nicolas; Hess, Philipp; Roque D'Orbcastel, Emmanuelle; Masseret, Estelle; Laabir, Mohamed. |
| Vulcanodinium rugosum, a recently described species, produces pinnatoxins. The IFR-VRU-01 strain, isolated from a French Mediterranean lagoon in 2010 and identified as the causative dinoflagellate contaminating mussels in the Ingril Lagoon (French Mediterranean) with pinnatoxin-G, was grown in an enriched natural seawater medium. We tested the effect of temperature and salinity on growth, pinnatoxin-G production and chlorophyll a levels of this dinoflagellate. These factors were tested in combinations of five temperatures (15, 20, 25, 30 and 35 °C) and five salinities (20, 25, 30, 35 and 40) at an irradiance of 100 µmol photon m−2 s−1. V. rugosum can grow at temperatures and salinities ranging from 20 °C to 30 °C and 20 to 40, respectively. The optimal... |
| Tipo: Text |
Palavras-chave: Vulcanodinium rugosum; Mediterranean Ingril Lagoon; Toxin production; Growth conditions; Temperature; Salinity. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00333/44429/44095.pdf |
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| Estevez, Pablo; Sibat, Manoella; Leão-martins, José Manuel; Tudó, Angels; Rambla-alegre, Maria; Aligizaki, Katerina; Diogène, Jorge; Gago-martinez, Ana; Hess, Philipp. |
| Over the last decade, knowledge has significantly increased on the taxonomic identity and distribution of dinoflagellates of the genera Gambierdiscus and Fukuyoa. Additionally, a number of hitherto unknown bioactive metabolites have been described, while the role of these compounds in ciguatera poisoning (CP) remains to be clarified. Ciguatoxins and maitotoxins are very toxic compounds produced by these dinoflagellates and have been described since the 1980s. Ciguatoxins are generally described as the main contributors to this food intoxication. Recent reports of CP in temperate waters of the Canary Islands (Spain) and the Madeira archipelago (Portugal) triggered the need for isolation and cultivation of dinoflagellates from these areas, and their... |
| Tipo: Text |
Palavras-chave: Maitotoxins; Ciguatoxins; Gambierdiscus; Fukuyoa; LC-MS; MS; HRMS; QToF; Ciguatera poisoning. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00629/74081/73530.pdf |
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| Soltani, Alex; Hess, Philipp; Dixon, Mike B; Boerlage, Siobhan F.e.; Anderson, Donald M; Newcombe, Gayle; House, Jenny; Ho, Lionel; Baker, Peter; Burch, Michael. |
| Tipo: Text |
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| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00407/51836/52437.pdf |
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| Reveillon, Damien; Abadie, Eric; Sechet, Veronique; Masseret, Estelle; Hess, Philipp; Amzil, Zouher. |
| The neurotoxin BMAA (β-N-methylamino-l-alanine) and its isomer DAB (2,4-diaminobutyric acid) have been detected in seafood worldwide, including in Thau lagoon (French Mediterranean Sea). A cluster of amyotrophic lateral sclerosis (ALS), a neurodegenerative disease associated with BMAA, has also been observed in this region. Mussels, periphyton (i.e. biofilms attached to mussels) and plankton were sampled between July 2013 and October 2014, and analyzed using HILIC-MS/MS. BMAA, DAB and AEG (N-(2-aminoethyl)glycine) were found in almost all the samples of the lagoon. BMAA and DAB were present at 0.58 and 0.83, 2.6 and 3.3, 4.0 and 7.2 μg g−1 dry weight in plankton collected with nets, periphyton and mussels, respectively. Synechococcus sp., Ostreococcus... |
| Tipo: Text |
Palavras-chave: BMAA; Diatoms; Chaetoceros sp.; Seston; Mytilus galloprovincialis; HILIC-MS/MS. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38607/37192.pdf |
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| Registros recuperados: 91 | |
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